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Browsing UNAHUR by Subject "Ciencias Biológicas"
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Publication Ancient varieties can help control weed density while preserving weed diversity(2023) Federico, Ana Ailén; Pimet, Eric; Arnaud Coffin, Chantal; Ducourtieux, WilfriedPublication Anthropogenic Infection of Domestic Cats With SARS-CoV-2 Alpha Variant B.1.1.7 Lineage in Buenos Aires(Frontiers Media, 2022) Pecora, Andrea; Malacari, Darío Amílcar; Mozgovoj, Marina Valeria; Díaz, María de los Ángeles; Peralta, Andrea Verónica; Cacciabue, Marco Polo Domingo; Puebla, Andrea Fabiana; Carusso, Cristian; Mundo, Silvia Leonor; González López Ledesma, María Mora; Gamarnik, Andrea VanesaSARS-CoV-2 reverse zoonosis, particularly to domestic animals, and the potential role of infected animals in perpetuating the spread of the virus is an issue of increasing concern. In this case report, we identified the natural infection of two cats by SARS-CoV-2, in Argentina, whose owner had been previously infected by SARS-CoV-2. Viral genetic material was detected in feline oropharyngeal (OP) and rectal (R) swab by RT-qPCR, and sequence analysis revealed that the virus infecting the owner and one cat were genetically similar. The alpha variant (B.1.1.7 lineage) was identified with a unique additional mutation, strongly suggesting human-to-cat route of transmission. This study reinforces the One Health concept and the importance of integrating human, animal, and environmental perspectives to promptly address relevant health issues.Publication Assessing small RNA profiles in potato diploid hybrid and its resynthesized allopolyploid reveals conserved abundance with distinct genomic distribution(2024) Zavallo, D.; Cara, N.; Leone, M.Publication Complete genome sequence of deformed wing virus and black queen cell virus isolated from honeybees (Apis mellifera) in Argentina(2024) González, F.; Ferrufino, C.; Dus Santos, M.J.; Carignano, H.A.Publication Cooperativity in regulation of membrane protein function : phenomenological analysis of the effects of pH and phospholipids(2023) Zerbetto De Palma, G; Recoulat Angelini, A.A.; Vitali, V.Publication Cost-Effective Method to Perform SARS-CoV-2 Variant Surveillance: Detection of Alpha, Gamma, Lambda, Delta, Epsilon, and Zeta in Argentina :(2021) Torres, C.; Mojsiejczuk L; Acuña, D.; Alexay, S.; Dus Santos, M.J.Publication Crop diversity improves carbon, nitrogen and soil biological functions in an agroecological system(2023) Ortíz, J.; Faggioli, V.; Zamora, M.; Boccolini, M.; Lorenzón, C.; Pegoraro, V.; Gabbarini, L.Publication Definition of the Emmallodera perlifera Species Group (Coleoptera: Tenebrionidae: Scotobiini) from Argentina with Descriptions of Two New Species(Polish Academy of Sciences, 2022) Silvestro, Violeta Ayelen; Macagno, Hilda Beatríz; Flores, Gustavo ErnestoThe genus Emmallodera Blanchard, 1842 (Tenebrionidae: Scotobiini) is widely distributed in western and southern Argentina and partially in southern Chile, inhabiting the biogeographic provinces Patagonian, Chacoan and Monte. The genus Emmallodera is well characterized within Scotobiini by the presence of a single spine on the profemur of its species, as well as by having pointed terminal antennomeres, and the tarsomeres loosely articulated to each other. Within the genus, E. perlifera Burmeister, 1875 is the largest species and is the only one characterized by the presence of tubercles on elytral intervals. Analysis of the recently collected specimens resulted in discovery of two new species (E. payunia Silvestro and Flores sp. nov. and E. andina Silvestro and Macagno sp. nov.), which share the main diagnostic features of E. perlifera but possess enough differences in elytral configuration and male genitalia to confirm that they are not conspecific. Under a deep morphological revision, it can be concluded that all of the three above-mentioned species constitute a well-characterized species-group inside the genus Emmallodera, while each of these taxa inhabits different biogeographic provinces, i.e.: Emmallodera perlifera (Monte and Chaco), E. payunia Silvestro and Flores sp. nov. (Patagonian) and E. andina Silvestro and Macagno sp. nov. (Puna). This paper includes taxonomic characterization of the group, designation of lectotype for E. perlifera, descriptions of the two new species, habitus photographs, illustrations of genital features and a distribution map.Publication Detección de SARS-CoV-2 en contactos estrechos asintomáticos de casos confirmados por diagnóstico molecular, provincia de Buenos Aires, Argentina(2021) Fischerman, L.; Ercole, R.; Mozgovoj, M.; Dus Santo, M.J.; Didier Garnham, M.; Lemes Lenivov, F.; Benvenutti, M.L.; Santana, L.Publication Detection of porcine circovirus 2, porcine parvovirus 1, and torque teno sus virus k2a in wild boars from northeastern Patagonia, Argentina(2023) De Maio, F.A.; Winter, M.; Abate, S.; Iglesias, N.G.Publication Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein(2024) Muzulin, P.; Brignone, J.; Iglesias, N.G.; Rodríguez, M.; Irazu, L.; García, J. B.; Levis, S.Publication Diversidad de cultivos mejora el carbono, nitrogeno y las funciones biológicas del suelo en un sistema agroecologico.(2023) Ortíz, J.; Faggioli, V.; Zamora, M.; Boccolini, M.; Lorenzón, C.; Pegoraro, V.; Gabbarini, L.Publication Enteroparasitosis en una población infantil del municipio de Hurlingham, Buenos Aires(Fundación Revista Medicina, 2022) de la Fourniere, Sofía Ana María.; Helman, María Elisa; Consiglio, Ezequiel; Etchemendi, Sebastián; Farber, Marisa Diana; López Arias, Ludmila SolLos parásitos intestinales tienen mayor impacto en las poblaciones infantiles ya que pueden, a largo plazo, conducir a un retraso en el crecimiento con alteración del desarrollo cognitivo. Sumado a esto, los entornos de vulnerabilidad social favorecen la transmisión y permanencia de estos parásitos en el ambiente. Métodos: En un estudio transversal realizado en el Municipio de Hurlingham (conurbano bonaerense) se estimó la prevalencia de enteroparasitosis y sus factores de riesgo asociados en una población infantil concurrente a Centros de Atención Primaria para la Salud locales mediante un análisis coproparasitológico, un test de Graham y una encuesta socioambiental y laboral. Resultados: Un total de 66 niñas (50.8%) y 64 niños (49.2%) entre 0 a 12 años fueron analizados. El 89.1% de los mayores de 4 años estaban escolarizados. En el 28.5% de los hogares la madre o el padre tenía trabajo formal y el 63.8% percibía la asignación universal por hijo (AUH). Se observaron condiciones de vulnerabilidad social en la población analizada, tales como: falta de acceso al agua de red y cloacas y un saneamiento ambiental deficiente. El diagnóstico parasitológico reveló un total de 75 casos [57.7% (IC 95%: 48.7-66.3)] de parasitosis. Los protistas fueron los parásitos más frecuentes y las especies más prevalentes: Blastocystis spp, Enterobius vermicularis, Giardia lamblia y Cryptosporidium sp. La presencia de parásitos estuvo asociada a la escolarización (p<0.01) y a la edad del niño (p<0.05). Discusión: Este fue el primer relevamiento sobre las enteroparasitosis en el municipio de Hurlingham y el contexto socioeconómico y ambiental donde se desarrollan.Publication Epidemias, salud y ciencia: interés y percepción de jóvenes de escuelas públicas del conurbano bonaerense antes del COVID-19(2021) Pedrosa, Juan Manuel; Petrucci, DiegoEn este trabajo describimos el grado de interés de jóvenes de escuelas públicas del Municipio de Hurlingham (Buenos Aires) respecto de aspectos relacionados con las epidemias y la salud, así como su percepción sobre la ciencia, la tecnología y los científicos. Los datos se recabaron en 2019 mediante el cuestionario del Proyecto ROSE y reflejan los intereses previos al COVID-19. Entre los resultados se evidencia una fuerte creencia en que la ciencia y la tecnología proporcionarán la cura para las enfermedades más preocupantes. Los ítems que despertaron más interés fueron: cómo realizar primeros auxilios y usar equipo médico básico; cáncer: lo que sabemos y cómo tratarlo; y cómo controlar las epidemias y enfermedades. Creemos que la información recabada ofrece un marco para cuantificar los cambios que la pandemia pueda generar.Publication Episodes of high tropospheric ozone reduce nodulation, seed production and quality in soybean (Glycine max (L.) merr.) on low fertility soilsBiancari, Lucio; Cerrota, Clara; Menéndez, Analía; Gundel, Pedro Emilio; Martínez Ghersa, María AlejandraDriven by human activities, air pollution and soil degradation are threatening food production systems. Rising ozone in the troposphere can affect several physiological processes in plants and their interaction with symbiotic microorganisms. Plant responses to ozone may depend on both soil fertility and the ontogenetic stage in which they are exposed. In this work, we studied the effects of ozone episodes and soil fertility on soybean plants. We analysed soybean plant responses in the production of aboveground and belowground biomass, structural and functional attributes of rhizobia, and seed production and quality. The experiment was performed with plants grown in two substrates with different fertility (commercial soil, and soil diluted (50%, v/v) with sand). Plants were exposed to acute episodes of ozone during vegetative and reproductive stages. We observed that ozone significantly reduced belowground biomass (z25%), nodule biomass (z30%), and biological nitrogen fixation (z21%). Plants exposed to ozone during reproductive stage growing in soil with reduced fertility had lower seed production (z10% lower) and seed protein (z12% lower). These responses on yield and quality can be explained by the observed changes in belowground biomass and nitrogen fixation. The negative impact of ozone on the symbiotic interaction with rhizobia, seed production and quality in soybean plants were greater in soils with reduced fertility. Our results indicate that food security could be at risk in the future if trends in ozone concentration and soil degradation processes continue to increase.Publication Nodal and churchill1 position the expression of a notch ligand during Xenopus germ layer segregation(2022) Favarolo, María Belén; Revinski, Diego R.; López, Silvia L.; Garavaglia, MatíasIn vertebrates, Nodal signaling plays a major role in endomesoderm induction, but germ layer delimitation is poorly understood. In avian embryos, the neural/mesoderm boundary is controlled by the transcription factor CHURCHILL1, presumably through the repressor ZEB2, but there is scarce knowledge about its role in other vertebrates. During amphibian gastrulation, Delta/Notch signaling refines germ layer boundaries in the marginal zone, but it is unknown the place this pathway occupies in the network comprising Churchill1 and Nodal. Here, we show that Xenopus churchill1 is expressed in the presumptive neuroectoderm at mid-blastula transition and during gastrulation, upregulates zeb2, prevents dll1 expression in the neuroectoderm, and favors neuroectoderm over endomesoderm development. Nodal signaling prevents dll1 expression in the endoderm but induces it in the presumptive mesoderm, from where it activates Notch1 and its target gene hes4 in the non-involuting marginal zone. We propose a model where Nodal and Churchill1 position Dll1/Notch1/Hes4 domains in the marginal zone, ensuring the delimitation between mesoderm and neuroectoderm.Publication Omicron Waves in Argentina : dynamics of SARS-CoV-2 Lineages BA.1, BA.2 and the Emerging BA.2.12.1 and BA.4/BA.5(2023) Torres, C.; Nabaes Jodar, M.; Acuña, D.; Montaño,.R.M.Z.; Culasso, A.C.A.; Aulicino, P.Publication P26 enhances baculovirus gene delivery by modulating the mammalian antiviral response(2023) Amalfi, S.; Plastine, M.D.; López, M.G.Publication PIP aquaporin pH-sensing is regulated by the length and charge of the C-terminal region(Wiley Blackwell, 2022) Scochera, Florencia Paola; Zerbetto de Palma, Gerardo Gabriel; Canessa Fortuna, Agustina; Chevriau, Jonathan; Toriano, Roxana Mabel; Soto, Gabriela CynthiaPlant PIP aquaporins play a central role in controlling plant water status. The current structural model for PIP pH-gating states that the main pH sensor is located in loopD and that all the mobile cytosolic elements participate in a complex interaction network that ensures the closed structure. However, the precise participation of the last part of the C-terminal domain (CT) in PIP pH gating remains unknown. This last part has not been resolved in PIP crystal structures and is a key difference between PIP1 and PIP2 paralogues. Here, by a combined experimental and computational approach, we provide data about the role of CT in pH gating of Beta vulgaris PIP. We demonstrate that the length of CT and the positive charge located among its last residues modulate the pH at which the open/closed transition occurs. We also postulate a molecular-based mechanism for the differential pH sensing in PIP homo- or heterotetramers by performing atomistic molecular dynamics simulations (MDS) on complete models of PIP tetramers. Our findings show that the last part of CT can affect the environment of loopD pH sensors in the closed state. Results presented herein contribute to the understanding of how the characteristics of CT in PIP channels play a crucial role in determining the pH at which water transport through these channels is blocked, highlighting the relevance of the differentially conserved very last residues in PIP1 and PIP2 paralogues.