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Browsing Publicaciones by Subject "Biotecnología Aplicada"
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Publication Ancient varieties can help control weed density while preserving weed diversity(2023) Federico, Ana Ailén; Pimet, Eric; Arnaud Coffin, Chantal; Ducourtieux, WilfriedPublication Anthropogenic Infection of Domestic Cats With SARS-CoV-2 Alpha Variant B.1.1.7 Lineage in Buenos Aires(Frontiers Media, 2022) Pecora, Andrea; Malacari, Darío Amílcar; Mozgovoj, Marina Valeria; Díaz, María de los Ángeles; Peralta, Andrea Verónica; Cacciabue, Marco Polo Domingo; Puebla, Andrea Fabiana; Carusso, Cristian; Mundo, Silvia Leonor; González López Ledesma, María Mora; Gamarnik, Andrea VanesaSARS-CoV-2 reverse zoonosis, particularly to domestic animals, and the potential role of infected animals in perpetuating the spread of the virus is an issue of increasing concern. In this case report, we identified the natural infection of two cats by SARS-CoV-2, in Argentina, whose owner had been previously infected by SARS-CoV-2. Viral genetic material was detected in feline oropharyngeal (OP) and rectal (R) swab by RT-qPCR, and sequence analysis revealed that the virus infecting the owner and one cat were genetically similar. The alpha variant (B.1.1.7 lineage) was identified with a unique additional mutation, strongly suggesting human-to-cat route of transmission. This study reinforces the One Health concept and the importance of integrating human, animal, and environmental perspectives to promptly address relevant health issues.Publication Antimicrobial Effect of Cannabidiol on Intracellular Mycobacterium tuberculosis(2024) Martinena, C.; Corleto, M.; Martínez, M.; Amiano, N.; García, V.; Maffia, Paulo César; Tateosian, N.Publication Assessing small RNA profiles in potato diploid hybrid and its resynthesized allopolyploid reveals conserved abundance with distinct genomic distribution(2024) Zavallo, D.; Cara, N.; Leone, M.Publication Comparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits(Elsevier, 2020) Polenta, Gustavo A.; Guidi, Silvina Mabel; Ambrosi, Vanina; Denoya, Gabriela I.Heat shock proteins (HSP) are synthesized in living tissues exposed to transient increase in temperature and play a central role in the protective response against heat and other stresses. In fruits, this response to heat treatment provides resistance to a physiological alteration known as chilling injury. Despite the physiological importance ofthis group of proteins, publications comparing different methodological alternatives for their analysis are rather scarce. In the present paper, we conducted a comparative study using different electrophoretic and immunological techniques to evaluate the HSP response in fruits. Proteins were extracted from tomato fruit exposed to an HSPinducing temperature (38 C) for different times (0, 3, 20, and 27 h). Different alternatives of analysis (SDS-PAGE, SDS-PAGE followed by IEF, Western blot, and dot blot) were performed, and their potential application discussed. The study was complemented with a practical application, in which tomatoes were subjected to heat and anaerobic treatments and then stored in a chill inducing temperature. This application evidences the relevance of knowing the level of proteins attained by stress treatments which correlates with the acquired tolerance.Publication Complete genome sequence of deformed wing virus and black queen cell virus isolated from honeybees (Apis mellifera) in Argentina(2024) González, Fernanda N.; Ferrufino, Cecilia; Dus Santos, María José; Carignano, Hugo A.We report the complete genome sequence of deformed wing virus and black queen cell virus isolated from Argentinean’s honeybees. These sequence data will be valuable for future research on the viral variants present in the country and the development of strategies to control the spread of these viruses in apiaries.Publication Cooperativity in regulation of membrane protein function : phenomenological analysis of the effects of pH and phospholipids(2023) Zerbetto De Palma, G; Recoulat Angelini, A.A.; Vitali, V.Publication Cost-Effective Method to Perform SARS-CoV-2 Variant Surveillance: Detection of Alpha, Gamma, Lambda, Delta, Epsilon, and Zeta in Argentina :(Frontiers Media, 2021) Torres, Carolina; Mojsiejczuk, Laura Noelia; Acuña, Dolores; Alexay, Sofía; Dus Santos, María José; Amadio, Ariel FernandoSARS-CoV-2 variants with concerning characteristics have emerged since the end of 2020. Surveillance of SARS-CoV-2 variants was performed on a total of 4,851 samples from the capital city and 10 provinces of Argentina, during 51 epidemiological weeks (EWs) that covered the end of the first wave and the ongoing second wave of the COVID-19 pandemic in the country (EW 44/2020 to EW 41/2021). The surveillance strategy was mainly based on Sanger sequencing of a Spike coding region that allows the identification of signature mutations associated with variants. In addition, whole-genome sequences were obtained from 637 samples. The main variants found were Gamma and Lambda, and to a lesser extent, Alpha, Zeta, and Epsilon, and more recently, Delta. Whereas, Gamma dominated in different regions of the country, both Gamma and Lambda prevailed in the most populated area, the metropolitan region of Buenos Aires. The lineages that circulated on the first wave were replaced by emergent variants in a term of a few weeks. At the end of the ongoing second wave, Delta began to be detected, replacing Gamma and Lambda. This scenario is consistent with the Latin American variant landscape, so far characterized by a concurrent increase in Delta circulation and a stabilization in the number of cases. The cost-effective surveillance protocol presented here allowed for a rapid response in a resource-limited setting, added information on the expansion of Lambda in South America, and contributed to the implementation of public health measures to control the disease spread in Argentina.Publication Crop diversity improves carbon, nitrogen and soil biological functions in an agroecological system(2023) Ortíz, J.; Faggioli, V.; Zamora, M.; Boccolini, M.; Lorenzón, C.; Pegoraro, V.; Gabbarini, L.Publication Detección de SARS-CoV-2 en contactos estrechos asintomáticos de casos confirmados por diagnóstico molecular, provincia de Buenos Aires, Argentina(2021) Fischerman, L.; Ercole, R.; Mozgovoj, M.; Dus Santo, M.J.; Didier Garnham, M.; Lemes Lenivov, F.; Benvenutti, M.L.; Santana, L.Publication Detection of porcine circovirus 2, porcine parvovirus 1, and torque teno sus virus k2a in wild boars from northeastern Patagonia, Argentina(2023) De Maio, F.A.; Winter, M.; Abate, S.; Iglesias, N.G.Publication Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein(2024) Muzulin, P.; Brignone, J.; Iglesias, N.G.; Rodríguez, M.; Irazu, L.; García, J. B.; Levis, S.Publication Development of a stable Sf9 insect cell line to produce VSV-G pseudotyped baculoviruses(2024) Plastine, M.D.P.; Amalfi, S.; López, M.G.Publication Diversidad de cultivos mejora el carbono, nitrogeno y las funciones biológicas del suelo en un sistema agroecologico.(2023) Ortíz, J.; Faggioli, V.; Zamora, M.; Boccolini, M.; Lorenzón, C.; Pegoraro, V.; Gabbarini, L.Publication Enhanced skin delivery of vismodegib-loaded rigid liposomes combined with ethosomes(2023) Aguayo Frías, E.T.; Maza Vega, D.; Calienni, María Natalia; Lillo, C.; Vázquez, D.S.; Del Valle Alonso, S.; Martinetti Montanari, Jorge AníbalPublication Evaluación del desempeño diagnóstico de una nueva qPCR para la confirmación de casos agudos y crónicos de babesiosis bovina por B. bovis(2023) Montenegro, V.; Valenzano, M.; Pérez, A,; López Arias, Ludmila; Guillemi, E.; Zimmer, P.; Sarmiento, N.; Pertile, C.Publication Green-synthesized silver nanoparticles using Aloe maculata extract as antibacterial agent for potential topical application(2023) Franceschinis, G.; Beverina, M.; Corleto, M.; Morena Sosa, A.; Lillo, Cristian; Aria Casará, L.; Del Valle Alonso, S.; Maffia, Paulo CésarPublication Kefir de agua, una bebida con microorganismos probióticos : desde la producción ancestral y artesanal hasta la comercialización, industrialización y regulación actuales(2022) Lino, M.; Moretti, A.; Moure, M.C.; Quiñoy, F.; Espósito, F.; Simonelli, N.; Medrano, M.; León Peláez, A.Publication Membrane permeability and antimicrobial peptides : much more than just making a hole : much more than just making a hole(2023) Espeche, J.C.; Varas, S.; Maturana, P.; Cutro, A.C.; Maffia, Paulo César; Hollman, A.