Person:
Martinetti Montanari, Jorge Aníbal

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14/05/1976
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Laboratorio de Nanosistemas de Aplicación Biotecnológica (LANSAB)
El Laboratorio de Nanosistemas de Aplicación Biotecnológica (LANSAB) está especializado en la encapsulación de fármacos, cosméticos, tópicos y activos naturales. Líneas de investigación: Nanotecnología aplicada a enfermedades cutáneas (cáncer de piel y leishmaniasis). Nanotecnología aplicada a formulaciones de uso veterinario. Nanotecnología aplicada a la salud del suelo. Desarrollo de nanocosméticos y nanorrepelentes.
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Investigador
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Martinetti Montanari
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Jorge Aníbal
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2020 - 20213
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    Biomaterial-based dressings as vehicle for chitosan-encapsulated cabreuva essential oil: Cytotoxicity and regenerative activity
    (Elsevier, 2020) Lamarra, Javier Andrés; Bucci, Paula Lorena; Giannuzzi, Leda; Rivero, Sandra G. M.; Pinotti, Adriana Noemí; Martinetti Montanari, Jorge Aníbal
    Chitosan-based emulsions functionalized with cabreuva essential oil (Myrocarpus fastigiatus) assembled with poly(vinyl alcohol) (PVA) were tailor-made to obtain a smooth, flexible and thin wound dressings capable for acting as a delivery vehicle. The cabreuva essential oil is composed mainly of trans-nerolidol, a natural compound with antimicrobial capacity that acts as a skin penetration enhancer. During the application of the dressing on skin explants, the penetration profile of cabreuva was analyzed through ex-vivo diffusion assays. Furthermore, the in-vivo essential oil delivery was followed by means of ATR-FTIR.The addition of chitosan-based emulsions produced modifications in the properties of the composite dressings associated with the water affinity and gas permeability as well as in the mechanical behavior. The dressing functionalized with cabreuva essential oil combined with chitosan proved its effectiveness against microorganisms such as S. aureus and S. epidermidis, capacity to produce cell regeneration after 24 h of contact time, and no cytotoxicity in HaCaT cells. The material developed could be used in superficial burns or minor wounds such as dressings due to its demonstrated functionality as controlled release devices of the cabreuva essential oil.
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    Small extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation
    (Cold Spring Harbor Laboratory Press, 2021) Biani, María Celeste; Lombardi, Antonella; Norris, Alessandra; Bucci, Paula Lorena; la Greca, Alejandro Damián; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo; Martinetti Montanari, Jorge Aníbal; Miriuka, Santiago Gabriel; Luzzani, Carlos Daniel
    Mesenchymal Stem Cells derived from induced Pluripotent Stem cells (iPSC-MSC) have become a promising alternative to classical Mesenchymal Stem Cells in regenerative medicine. Their properties -as immunomodulatory and regenerative capacities-are in part due to the secretion of Extracellular Vesicles (EVs). Small EVs (sEVs) with sizes that range from 50 to 120 nm contain proteins, lipids, and nucleic acids that exert a role in cellular communication. Their content will depend on the cell of origin and its physiological state, thus the message they convey might change in response to changes in cellular conditions. In particular, the DNA damage response (DDR) has been reported to modulate sEVs secretion. In this work, we analyze how UV-C radiation upon iPSC-MSC alter sEVs secretion, cargo and bystander effect. Here, we confirm that UV-C radiation causes DDR in a dose dependent manner. In addition, we found that UV-C induced stress did not modulate the expression of genes that participate in sEVs biogenesis pathway. Consequently, we found that the amount of sEVs secreted by radiated and non-irradiated cells remained stable. However, sEVs from radiated cells were unable to promote cell migration in their target cells. Moreover, a label-free proteomic analysis revealed that UV-C induced DDR produces sEVs with an altered cargo, rich in migration-inhibiting proteins, and resulting in a less stromal-oriented repertoire.
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    Paclitaxel and curcumin co-loaded mixed micelles. Improving in vitro efficacy and reducing toxicity against Abraxane®
    (2021) Riedel, Jennifer Denise; Bernabeu, Ezequiel Adrián; Calabró López, María Valeria.; Calienni, María Natalia; Lázaro Martínez, Juan Manuel; Prieto, María Jimena; González, Lorena; Martínez, Carolina Soledad; Alonso Del Valle, Silvia; Martinetti Montanari, Jorge Aníbal; Evelson, Pablo Andrés; Chiappetta, Diego Andrés; Moretton, Marcela Analía
    Vismodegib is a first-in-class inhibitor for advanced basal cell carcinoma treatment. Its dailyoral doses present a high distribution volume and several side effects. We evaluated its skin penetration loaded in diverse nanosystems as potential strategies to reduce side effects and drug quantities. Ultradeformable liposomes, ethosomes, colloidal liquid crystals, and dendrimers were able to transport Vismodegib to deep skin layers, while polymeric micelles failed at this. As lipidic systems were the most effective, we assessed the in vitro and in vivo toxicity of Vismodegib-loaded ultradeformable liposomes, apoptosis, and cellular uptake. Vismodegib emerges as a versatile drug that can be loaded in several delivery systems for topical application. These findings may be also useful for the consideration of topical delivery of other drugs with a low water solubility.