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Antimicrobial Effect of Cannabidiol on Intracellular Mycobacterium tuberculosis
(2024) Martinena, C.; Corleto, M.; Martínez, M.; Amiano, N.; García, V.; Maffia, Paulo César; Tateosian, N.
Comparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits
(Elsevier, 2020) Polenta, Gustavo A.; Guidi, Silvina Mabel; Ambrosi, Vanina; Denoya, Gabriela I.
Heat shock proteins (HSP) are synthesized in living tissues exposed to transient increase in temperature and play a central role in the protective response against heat and other stresses. In fruits, this response to heat treatment provides resistance to a physiological alteration known as chilling injury. Despite the physiological importance ofthis group of proteins, publications comparing different methodological alternatives for their analysis are rather scarce. In the present paper, we conducted a comparative study using different electrophoretic and immunological techniques to evaluate the HSP response in fruits. Proteins were extracted from tomato fruit exposed to an HSPinducing temperature (38 C) for different times (0, 3, 20, and 27 h). Different alternatives of analysis (SDS-PAGE, SDS-PAGE followed by IEF, Western blot, and dot blot) were performed, and their potential application discussed. The study was complemented with a practical application, in which tomatoes were subjected to heat and anaerobic treatments and then stored in a chill inducing temperature. This application evidences the relevance of knowing the level of proteins attained by stress treatments which correlates with the acquired tolerance.
Development of a stable Sf9 insect cell line to produce VSV-G pseudotyped baculoviruses
(2024) Plastine, M.D.P.; Amalfi, S.; López, M.G.
Enhanced skin delivery of vismodegib-loaded rigid liposomes combined with ethosomes
(2023) Aguayo Frías, E.T.; Maza Vega, D.; Calienni, María Natalia; Lillo, C.; Vázquez, D.S.; Del Valle Alonso, S.; Martinetti Montanari, Jorge Aníbal
Evaluación del desempeño diagnóstico de una nueva qPCR para la confirmación de casos agudos y crónicos de babesiosis bovina por B. bovis
(2023) Montenegro, V.; Valenzano, M.; Pérez, A,; López Arias, Ludmila; Guillemi, E.; Zimmer, P.; Sarmiento, N.; Pertile, C.
Green-synthesized silver nanoparticles using Aloe maculata extract as antibacterial agent for potential topical application
(2023) Franceschinis, G.; Beverina, M.; Corleto, M.; Morena Sosa, A.; Lillo, Cristian; Aria Casará, L.; Del Valle Alonso, S.; Maffia, Paulo César
Membrane permeability and antimicrobial peptides : much more than just making a hole : much more than just making a hole
(2023) Espeche, J.C.; Varas, S.; Maturana, P.; Cutro, A.C.; Maffia, Paulo César; Hollman, A.
Nanomaterials for diagnosis, treatment, and prevention of human cutaneous leishmaniasis : a review
(2023) Maza Vega, D.; Meglio, M.; Del Valle Alonso, S.; Alvira, F.; Martinetti Montanari, Jorge Aníbal
Paclitaxel and curcumin co-loaded mixed micelles. Improving in vitro efficacy and reducing toxicity against Abraxane®
(2021) Riedel, Jennifer Denise; Bernabeu, Ezequiel Adrián; Calabró López, María Valeria.; Calienni, María Natalia; Lázaro Martínez, Juan Manuel; Prieto, María Jimena; González, Lorena; Martínez, Carolina Soledad; Alonso Del Valle, Silvia; Martinetti Montanari, Jorge Aníbal; Evelson, Pablo Andrés; Chiappetta, Diego Andrés; Moretton, Marcela Analía
Vismodegib is a first-in-class inhibitor for advanced basal cell carcinoma treatment. Its dailyoral doses present a high distribution volume and several side effects. We evaluated its skin penetration loaded in diverse nanosystems as potential strategies to reduce side effects and drug quantities. Ultradeformable liposomes, ethosomes, colloidal liquid crystals, and dendrimers were able to transport Vismodegib to deep skin layers, while polymeric micelles failed at this. As lipidic systems were the most effective, we assessed the in vitro and in vivo toxicity of Vismodegib-loaded ultradeformable liposomes, apoptosis, and cellular uptake. Vismodegib emerges as a versatile drug that can be loaded in several delivery systems for topical application. These findings may be also useful for the consideration of topical delivery of other drugs with a low water solubility.
Prevalencia y distribución geográfica de las geohelmintiasis en Argentina : revisión sistemática y metaanálisis
(Sociedad Argentina de Protozoología, 2023)
Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin
(2023) Gómez, G.E.; Calienni, María Natalia; Alonso del Valle, S.; Alvira, F.C.; Martinetti, Jorge Aníbal
Review of antiviral peptides for use against zoonotic and selected non-zoonotic viruses
(Elsevier Science, 2021) Hollmann, Axel; Cardoso, Nancy Patricia; Espeche, Juan Carlos; Maffia, Paulo César
Viruses remain one of the leading causes of animal and human disease. Some animal viral infections spread sporadically to human populations, posing a serious health risk. Particularly the emerging viral zoonotic diseases such as the novel, zoonotic coronavirus represent an actual challenge for the scientific and medical community. Besides human health risks, some animal viral infections, although still not zoonotic, represent important economic loses to the livestock industry. Viral infections pose a genuine concern for which there has been an increasing interest for new antiviral molecules. Among these novel compounds, antiviral peptides have been proposed as promising therapeutic options, not only for the growing body of evidence showing hopeful results but also due to the many adverse effects of chemical-based drugs. Here we review the current progress, key targets and considerations for the development of antiviral peptides (AVPs). The review summarizes the state of the art of the AVPs tested in zoonotic (coronaviruses, Rift Valley fever viruses, Eastern Equine Encephalitis Virus, Dengue and Junín virus) and also non-zoonotic farm animal viruses (avian and cattle viruses). Their molecular target, amino acid sequence and mechanism of action are summarized and reviewed. Antiviral peptides are currently on the cutting edge since they have been reported to display anti-coronavirus activity. Particularly, the review will discuss the specific mode of action of AVPs that specifically inhibit the fusion of viral and host-cell membranes for SARS-CoV-2, showing in detail some important features of the fusion inhibiting peptides that target the spike protein of these risky viruses
Rheological and Viscoelastic Analysis of Hybrid Formulations for Topical Application
(2023) Calienni, María Natalia; Martínez, L.M.; Izquierdo, M.C.; Del Valle, Alonso, S.; Martinetti, Jorge Aníbal
Rheological, Physical, and Spectroscopical Characterization of Gamma-Irradiated Albumin Nanoparticles Loaded with Anthocyanin
(2023) Cándido, S.L.; Siri, M.; Achili, E.; Moreno, J.C.; Lillo, C.; Risso, P.; Bodycomb, J.; Martínez, L.
Small extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation
(Cold Spring Harbor Laboratory Press, 2021) Biani, María Celeste; Lombardi, Antonella; Norris, Alessandra; Bucci, Paula Lorena; la Greca, Alejandro Damián; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo; Martinetti Montanari, Jorge Aníbal; Miriuka, Santiago Gabriel; Luzzani, Carlos Daniel
Mesenchymal Stem Cells derived from induced Pluripotent Stem cells (iPSC-MSC) have become a promising alternative to classical Mesenchymal Stem Cells in regenerative medicine. Their properties -as immunomodulatory and regenerative capacities-are in part due to the secretion of Extracellular Vesicles (EVs). Small EVs (sEVs) with sizes that range from 50 to 120 nm contain proteins, lipids, and nucleic acids that exert a role in cellular communication. Their content will depend on the cell of origin and its physiological state, thus the message they convey might change in response to changes in cellular conditions. In particular, the DNA damage response (DDR) has been reported to modulate sEVs secretion. In this work, we analyze how UV-C radiation upon iPSC-MSC alter sEVs secretion, cargo and bystander effect. Here, we confirm that UV-C radiation causes DDR in a dose dependent manner. In addition, we found that UV-C induced stress did not modulate the expression of genes that participate in sEVs biogenesis pathway. Consequently, we found that the amount of sEVs secreted by radiated and non-irradiated cells remained stable. However, sEVs from radiated cells were unable to promote cell migration in their target cells. Moreover, a label-free proteomic analysis revealed that UV-C induced DDR produces sEVs with an altered cargo, rich in migration-inhibiting proteins, and resulting in a less stromal-oriented repertoire.
The Topical Nanodelivery of Vismodegib Enhances Its Skin Penetration and Performance In Vitro While Reducing Its Toxicity In Vivo
(2021) Calienni, María Natalia; Vega, D.; Temprana, C.F.I.; Prieto, M.J.; Martinetti Montanari, Jorge Aníbal
Torque teno sus virus k2a (TTSuVk2a) in wild boars from northeastern Patagonia, Argentina
(2024) De Maio, F.A.; Winter, M.; Abate, S.; Iglesias, N.G.
Vismodegib in PAMAM-dendrimers for potential theragnosis in skin cancer
(Elsevier, 2022) Ybarra, David Emanuel; Calienni, María Natalia; Ramirez, Luis Felipe Barraza; Aguayo Frias, Eliana Taís; Lillo, Rolando Cristian Rodrigo; Alonso Del Valle, Silvia; Martinetti Montanari, Jorge Aníbal; Alvira, Fernando Carlos
Vismodegib (VDG) is an antineoplastic, a first-in-class Hedgehog signaling pathway inhibitor, indicated to treat locally advanced or metastatic basal cell carcinoma. Treatment with this drug was approved in 2012 by the US-FDA for oral administration (dose of 150 mg per day) in patients with a refusal of radiotherapy or surgery. However, it presents side effects that influence patient adherence to treatment. Polyamidoamine (PAMAM) dendrimers (D) are promising drug-delivery systems with high water solubility. Additionally, they can penetrate the skin barrier. In this work, we used amine-terminated (DG4.0) and carboxy-terminated (DG4.5) dendrimers of generation 4.0 and 4.5, respectively. We demonstrated that the complexation of VDG with dendrimers (D:VDG complexes) increased its concentration in the aqueous medium. We carried out characterization studies of the complexes to understand how dendrimers interact with VDG, and we found the optimal molar ratios of complexation.
Wastewater surveillance of enteric viruses in eastern Argentina : high rates of detection and first report of NoV GI.5 and GII.20
(Elsevier, 2024) Frydman, Camila Ayelén; Miño, S.; Iglesias, N.G.; Carballeda, J.M.; Simari, Milagros Belén; Pisano, María Belén; Dus Santos, María José; Mozgovoj, Marina Valeria
Individuals infected with enteric viruses excrete them in their feces for an extended period, whether symptomatic or asymptomatic. This characteristic, combined with the capability of these viruses to persist in the environment, forms the core of our research. The objective of our study was to investigate the presence of viruses associated with diarrhea and hepatitis: Hepatitis A virus (HAV), Hepatitis E virus (HEV), Norovirus GI (NoV GI), Norovirus GII (NoV GII), and Rotavirus (RV) by RT-real time PCR, in untreated wastewater samples (n=100) collected in the period July 2020 to August 2021 from two low-income neighborhoods in the district of La Plata, Buenos Aires, Argentina. Globally, the percentage of positive samples was 25 % for HEV, 27 % for RV, 14 % for NoV GII, 1 % for NoV GI, and no detectable samples were found for HAV. HEV, RV, and NoV GII were detected in most of the studied months, with the highest detection rates of RV and NoV GII during the winter season. Regarding RV positive samples, the gene encoding the VP8* protein of three samples was sequenced and phylogenetic analysis revealed that the detected strains belonged to genotypes P[8] and P[3]. Additionally, four NoV strains were also genetically characterized by amplifying a fragment corresponding to the ORF-1/ORF-2 junction region. The identified strains were NoV GI.5, NoV GII.4, NoV GII.17 and NoV GII.20. Our results provide relevant information and serve as scientific evidence of the importance of considering wastewater analysis as a feasible strategy to determine the circulation of enteric viruses in the population, with the further benefit of predicting emerging strains. Moreover, this study represents the first report of the circulation of NoV GII.20 and GI.5 genotypes in our country.

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