Artículos de revistas

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Comparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits
(Elsevier, 2020) Polenta, Gustavo A.; Guidi, Silvina Mabel; Ambrosi, Vanina; Denoya, Gabriela I.
Heat shock proteins (HSP) are synthesized in living tissues exposed to transient increase in temperature and play a central role in the protective response against heat and other stresses. In fruits, this response to heat treatment provides resistance to a physiological alteration known as chilling injury. Despite the physiological importance ofthis group of proteins, publications comparing different methodological alternatives for their analysis are rather scarce. In the present paper, we conducted a comparative study using different electrophoretic and immunological techniques to evaluate the HSP response in fruits. Proteins were extracted from tomato fruit exposed to an HSPinducing temperature (38 C) for different times (0, 3, 20, and 27 h). Different alternatives of analysis (SDS-PAGE, SDS-PAGE followed by IEF, Western blot, and dot blot) were performed, and their potential application discussed. The study was complemented with a practical application, in which tomatoes were subjected to heat and anaerobic treatments and then stored in a chill inducing temperature. This application evidences the relevance of knowing the level of proteins attained by stress treatments which correlates with the acquired tolerance.
Desórdenes de la conciencia en niños
(Red Latinoamerica y del Caribe de Bioética. Unesco, 2019) Gorga Christin, Marcelo Fabián
Actualmente se reconoce, especialmente en niños, la dificultad vinculada al establecimiento deldiagnóstico y pronóstico en pacientes con desórdenes dela conciencia como el estado vegetativo. Las dificultades respecto al pronóstico surgen de la presencia de casos con recuperación de laconciencia luego del tiempo establecido para diagnosticar un estado vegetativo permanente. Estas evidencias provocaron el cuestionamiento de esta categoría diagnóstica, asociada a una condición de irreversibilidad. Sin embargo, sigue siendo imperativo preservar el derecho a una muerte digna cuando las intervenciones médicas adquieren desproporción en relación a la posibilidad y/o probabilidad de beneficio terapéutico. Nos preguntamos acerca de las implicancias bioéticas y neuroéticas de estos hechos. Planteamos como hipótesis que el principio ético rector que permite respetar los derechos de los niños con estos desórdenes es el de proporcionalidad terapéutica, más allá de las categorías diagnósticas consensuadas en el ámbito médico.
Paclitaxel and curcumin co-loaded mixed micelles. Improving in vitro efficacy and reducing toxicity against Abraxane®
(2021) Riedel, Jennifer Denise; Bernabeu, Ezequiel Adrián; Calabró López, María Valeria.; Calienni, María Natalia; Lázaro Martínez, Juan Manuel; Prieto, María Jimena; González, Lorena; Martínez, Carolina Soledad; Alonso Del Valle, Silvia; Martinetti Montanari, Jorge Aníbal; Evelson, Pablo Andrés; Chiappetta, Diego Andrés; Moretton, Marcela Analía
Vismodegib is a first-in-class inhibitor for advanced basal cell carcinoma treatment. Its dailyoral doses present a high distribution volume and several side effects. We evaluated its skin penetration loaded in diverse nanosystems as potential strategies to reduce side effects and drug quantities. Ultradeformable liposomes, ethosomes, colloidal liquid crystals, and dendrimers were able to transport Vismodegib to deep skin layers, while polymeric micelles failed at this. As lipidic systems were the most effective, we assessed the in vitro and in vivo toxicity of Vismodegib-loaded ultradeformable liposomes, apoptosis, and cellular uptake. Vismodegib emerges as a versatile drug that can be loaded in several delivery systems for topical application. These findings may be also useful for the consideration of topical delivery of other drugs with a low water solubility.
Review of antiviral peptides for use against zoonotic and selected non-zoonotic viruses
(Elsevier Science, 2021) Hollmann, Axel; Cardoso, Nancy Patricia; Espeche, Juan Carlos; Maffia, Paulo César
Viruses remain one of the leading causes of animal and human disease. Some animal viral infections spread sporadically to human populations, posing a serious health risk. Particularly the emerging viral zoonotic diseases such as the novel, zoonotic coronavirus represent an actual challenge for the scientific and medical community. Besides human health risks, some animal viral infections, although still not zoonotic, represent important economic loses to the livestock industry. Viral infections pose a genuine concern for which there has been an increasing interest for new antiviral molecules. Among these novel compounds, antiviral peptides have been proposed as promising therapeutic options, not only for the growing body of evidence showing hopeful results but also due to the many adverse effects of chemical-based drugs. Here we review the current progress, key targets and considerations for the development of antiviral peptides (AVPs). The review summarizes the state of the art of the AVPs tested in zoonotic (coronaviruses, Rift Valley fever viruses, Eastern Equine Encephalitis Virus, Dengue and Junín virus) and also non-zoonotic farm animal viruses (avian and cattle viruses). Their molecular target, amino acid sequence and mechanism of action are summarized and reviewed. Antiviral peptides are currently on the cutting edge since they have been reported to display anti-coronavirus activity. Particularly, the review will discuss the specific mode of action of AVPs that specifically inhibit the fusion of viral and host-cell membranes for SARS-CoV-2, showing in detail some important features of the fusion inhibiting peptides that target the spike protein of these risky viruses
Small extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation
(Cold Spring Harbor Laboratory Press, 2021) Biani, María Celeste; Lombardi, Antonella; Norris, Alessandra; Bucci, Paula Lorena; la Greca, Alejandro Damián; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo; Martinetti Montanari, Jorge Aníbal; Miriuka, Santiago Gabriel; Luzzani, Carlos Daniel
Mesenchymal Stem Cells derived from induced Pluripotent Stem cells (iPSC-MSC) have become a promising alternative to classical Mesenchymal Stem Cells in regenerative medicine. Their properties -as immunomodulatory and regenerative capacities-are in part due to the secretion of Extracellular Vesicles (EVs). Small EVs (sEVs) with sizes that range from 50 to 120 nm contain proteins, lipids, and nucleic acids that exert a role in cellular communication. Their content will depend on the cell of origin and its physiological state, thus the message they convey might change in response to changes in cellular conditions. In particular, the DNA damage response (DDR) has been reported to modulate sEVs secretion. In this work, we analyze how UV-C radiation upon iPSC-MSC alter sEVs secretion, cargo and bystander effect. Here, we confirm that UV-C radiation causes DDR in a dose dependent manner. In addition, we found that UV-C induced stress did not modulate the expression of genes that participate in sEVs biogenesis pathway. Consequently, we found that the amount of sEVs secreted by radiated and non-irradiated cells remained stable. However, sEVs from radiated cells were unable to promote cell migration in their target cells. Moreover, a label-free proteomic analysis revealed that UV-C induced DDR produces sEVs with an altered cargo, rich in migration-inhibiting proteins, and resulting in a less stromal-oriented repertoire.
Vismodegib in PAMAM-dendrimers for potential theragnosis in skin cancer
(Elsevier, 2022) Ybarra, David Emanuel; Calienni, María Natalia; Ramirez, Luis Felipe Barraza; Aguayo Frias, Eliana Taís; Lillo, Rolando Cristian Rodrigo; Alonso Del Valle, Silvia; Martinetti Montanari, Jorge Aníbal; Alvira, Fernando Carlos
Vismodegib (VDG) is an antineoplastic, a first-in-class Hedgehog signaling pathway inhibitor, indicated to treat locally advanced or metastatic basal cell carcinoma. Treatment with this drug was approved in 2012 by the US-FDA for oral administration (dose of 150 mg per day) in patients with a refusal of radiotherapy or surgery. However, it presents side effects that influence patient adherence to treatment. Polyamidoamine (PAMAM) dendrimers (D) are promising drug-delivery systems with high water solubility. Additionally, they can penetrate the skin barrier. In this work, we used amine-terminated (DG4.0) and carboxy-terminated (DG4.5) dendrimers of generation 4.0 and 4.5, respectively. We demonstrated that the complexation of VDG with dendrimers (D:VDG complexes) increased its concentration in the aqueous medium. We carried out characterization studies of the complexes to understand how dendrimers interact with VDG, and we found the optimal molar ratios of complexation.

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