Publication:
Small extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation

Simple item page
dc.contributor.authorBiani, María Celeste
dc.contributor.authorLombardi, Antonella
dc.contributor.authorNorris, Alessandra
dc.contributor.authorBucci, Paula Lorena
dc.contributor.authorla Greca, Alejandro Damián
dc.contributor.authorWaisman, Ariel
dc.contributor.authorMoro, Lucía Natalia
dc.contributor.authorSevlever, Gustavo
dc.contributor.authorMartinetti Montanari, Jorge Aníbal
dc.contributor.authorMiriuka, Santiago Gabriel
dc.contributor.authorLuzzani, Carlos Daniel
dc.date.accessioned2025-01-06T18:32:58Z
dc.date.available2025-01-06T18:32:58Z
dc.date.issued2021
dc.description.abstractMesenchymal Stem Cells derived from induced Pluripotent Stem cells (iPSC-MSC) have become a promising alternative to classical Mesenchymal Stem Cells in regenerative medicine. Their properties -as immunomodulatory and regenerative capacities-are in part due to the secretion of Extracellular Vesicles (EVs). Small EVs (sEVs) with sizes that range from 50 to 120 nm contain proteins, lipids, and nucleic acids that exert a role in cellular communication. Their content will depend on the cell of origin and its physiological state, thus the message they convey might change in response to changes in cellular conditions. In particular, the DNA damage response (DDR) has been reported to modulate sEVs secretion. In this work, we analyze how UV-C radiation upon iPSC-MSC alter sEVs secretion, cargo and bystander effect. Here, we confirm that UV-C radiation causes DDR in a dose dependent manner. In addition, we found that UV-C induced stress did not modulate the expression of genes that participate in sEVs biogenesis pathway. Consequently, we found that the amount of sEVs secreted by radiated and non-irradiated cells remained stable. However, sEVs from radiated cells were unable to promote cell migration in their target cells. Moreover, a label-free proteomic analysis revealed that UV-C induced DDR produces sEVs with an altered cargo, rich in migration-inhibiting proteins, and resulting in a less stromal-oriented repertoire.
dc.formatapplication/pdf
dc.identifier.citationBiani, María Celeste; Lombardi, Antonella; Norris, Alessandra; Bucci, Paula Lorena; la Greca, Alejandro Damián; et al.; Small extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation; Cold Spring Harbor Laboratory Press; bioRxiv; 9-2021; 1-14
dc.identifier.issn2692-8205
dc.identifier.urihttps://repositorio.unahur.edu.ar/handle/123456789/383
dc.journal.pagination1-14
dc.journal.titlebioRxiv
dc.language.isoeng
dc.publisherCold Spring Harbor Laboratory Press
dc.relation.alternativeidhttps://doi.org/10.1101/2021.09.27.461979
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licenseAttribution-NonCommercial-ShareAlike 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.ocde1Ciencias Médicas y de la Saludes
dc.subject.ocde2Biotecnología en Salud
dc.subject.unahurUna salud. Biotecnologíaes
dc.titleSmall extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation
dc.typeinfo:eu-repo/semantics/article
dc.type.snrdinfo:ar-repo/semantics/artículo
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
relation.isAuthorOfPublicationc619b3e2-c534-47fb-aada-ccd412898176
relation.isAuthorOfPublication.latestForDiscoveryc619b3e2-c534-47fb-aada-ccd412898176
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_2021_Biani_et-al_Small_extracellular_vesicles.pdf
Size:
24.82 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.65 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
Artículos de revistas