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PIP aquaporin pH-sensing is regulated by the length and charge of the C-terminal region

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dc.contributor.authorScochera, Florencia Paola
dc.contributor.authorZerbetto de Palma, Gerardo Gabriel
dc.contributor.authorCanessa Fortuna, Agustina
dc.contributor.authorChevriau, Jonathan
dc.contributor.authorToriano, Roxana Mabel
dc.contributor.authorSoto, Gabriela Cynthia
dc.date.accessioned2025-01-30T00:05:43Z
dc.date.available2025-01-30T00:05:43Z
dc.date.issued2022
dc.description.abstractPlant PIP aquaporins play a central role in controlling plant water status. The current structural model for PIP pH-gating states that the main pH sensor is located in loopD and that all the mobile cytosolic elements participate in a complex interaction network that ensures the closed structure. However, the precise participation of the last part of the C-terminal domain (CT) in PIP pH gating remains unknown. This last part has not been resolved in PIP crystal structures and is a key difference between PIP1 and PIP2 paralogues. Here, by a combined experimental and computational approach, we provide data about the role of CT in pH gating of Beta vulgaris PIP. We demonstrate that the length of CT and the positive charge located among its last residues modulate the pH at which the open/closed transition occurs. We also postulate a molecular-based mechanism for the differential pH sensing in PIP homo- or heterotetramers by performing atomistic molecular dynamics simulations (MDS) on complete models of PIP tetramers. Our findings show that the last part of CT can affect the environment of loopD pH sensors in the closed state. Results presented herein contribute to the understanding of how the characteristics of CT in PIP channels play a crucial role in determining the pH at which water transport through these channels is blocked, highlighting the relevance of the differentially conserved very last residues in PIP1 and PIP2 paralogues.
dc.description.filiationFil: Scochera, Florencia Paola. Universidad Nacional de la Plata. Facultad de Ingeniería. Departamento de Fisicomatemática; Argentina.
dc.description.filiationFil: Zerbetto de Palma, Gerardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.filiationFil: Canessa Fortuna, Agustina. Universidad de Buenos Aires; Argentina
dc.description.filiationFil: Chevriau, Jonathan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.filiationFil: Toriano, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.description.filiationFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
dc.format.mimetypeapplication/pdf
dc.identifier.citationScochera, Florencia Paola; Zerbetto de Palma, Gerardo Gabriel; Canessa Fortuna, Agustina; Chevriau, Jonathan; Toriano, Roxana Mabel; et al. (2022) PIP aquaporin pH-sensing is regulated by the length and charge of the C-terminal region. Febs Journal; 289 (1) pp. 246-261
dc.identifier.doihttp://dx.doi.org/10.1007/s00248-022-02119-0
dc.identifier.issn1742-464X
dc.identifier.urihttps://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16134
dc.identifier.urihttps://repositorio.unahur.edu.ar/handle/123456789/515
dc.journal.number1
dc.journal.pagination246-261
dc.journal.titleFEBS Journal
dc.journal.volume289
dc.language.isoeng
dc.publisherWiley Blackwell
dc.rights.licenseinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.ocde1Ciencias Exactas y Naturales
dc.subject.ocde2Ciencias Biológicas
dc.subject.unahurBiotecnología Aplicada
dc.titlePIP aquaporin pH-sensing is regulated by the length and charge of the C-terminal region
dc.typeinfo:eu-repo/semantics/article
dc.type.oaireinfo:eu-repo/semantics/article
dc.type.snrdinfo:ar-repo/semantics/artículo
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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