Publication:
Comparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits

Simple item page
dc.contributor.authorPolenta, Gustavo A.es
dc.contributor.authorGuidi, Silvina Mabeles
dc.contributor.authorAmbrosi, Vaninaes
dc.contributor.authorDenoya, Gabriela I.es
dc.date.accessioned2024-11-15T13:46:04Z
dc.date.available2024-11-15T13:46:04Z
dc.date.issued2020
dc.description.abstractHeat shock proteins (HSP) are synthesized in living tissues exposed to transient increase in temperature and play a central role in the protective response against heat and other stresses. In fruits, this response to heat treatment provides resistance to a physiological alteration known as chilling injury. Despite the physiological importance ofthis group of proteins, publications comparing different methodological alternatives for their analysis are rather scarce. In the present paper, we conducted a comparative study using different electrophoretic and immunological techniques to evaluate the HSP response in fruits. Proteins were extracted from tomato fruit exposed to an HSPinducing temperature (38 C) for different times (0, 3, 20, and 27 h). Different alternatives of analysis (SDS-PAGE, SDS-PAGE followed by IEF, Western blot, and dot blot) were performed, and their potential application discussed. The study was complemented with a practical application, in which tomatoes were subjected to heat and anaerobic treatments and then stored in a chill inducing temperature. This application evidences the relevance of knowing the level of proteins attained by stress treatments which correlates with the acquired tolerance.en
dc.description.filiationFil: Polenta, Gustavo Alberto. Universidad Nacional de Hurlingham. Instituto en Biotecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Universidad de Morón. Facultad de Agronomía y Ciencias Agroalimentarias; Argentina.es
dc.description.filiationFil: Guidi, Silvina Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Universidad de Morón. Facultad de Agronomía y Ciencias Agroalimentarias; Argentina.es
dc.description.filiationFil: Ambrosi, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Universidad de Morón. Facultad de Agronomía y Ciencias Agroalimentarias; Argentina.es
dc.description.filiationFil: Denoya, Gabriela Inés. Universidad Nacional de Hurlingham. Instituto en Biotecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es
dc.formatapplication/pdf
dc.identifier.citationPolenta, G.A., Guidi, S.M., Ambrosi, V. & Denoya, G.I. Comparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits: application to tomatoes subjected to stress treatments. (2020) Current Research in Food Science, 3, 329-338.es
dc.identifier.doihttps://doi.org/10.1016/j.crfs.2020.09.002
dc.identifier.issn2665-9271
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S2665927120300332
dc.identifier.urihttps://repositorio.unahur.edu.ar/handle/123456789/346
dc.journal.pagination329-338
dc.journal.titleCurrent Research in Food Science
dc.language.isoen
dc.publisherElsevier
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2665927120300332
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceCurrent Research in Food Science
dc.subject.ocde1Ciencias Médicas y de la Saludes
dc.subject.ocde1Biotecnología en Saludes
dc.subject.unahurBiotecnología Aplicadaes
dc.titleComparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits
dc.title.subtitleapplication to tomatoes subjected to stress treatments
dc.type.oaireinfo:ar-repo/semantics/article
dc.type.snrdinfo:ar-repo/semantics/artículo
dc.type.unahurartículo de revista
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_2020_Polenta_Comparison_different_analytical.pdf
Size:
1.22 MB
Format:
Adobe Portable Document Format
Download
Collections
Artículos de revistas