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Wastewater surveillance of enteric viruses in eastern Argentina : high rates of detection and first report of NoV GI.5 and GII.20

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dc.contributor.authorFrydman, Camila Ayelén
dc.contributor.authorMiño, S.
dc.contributor.authorIglesias, N.G.
dc.contributor.authorCarballeda, J.M.
dc.contributor.authorSimari, Milagros Belén
dc.contributor.authorPisano, María Belén
dc.contributor.authorDus Santos, María José
dc.contributor.authorMozgovoj, Marina Valeria
dc.date.accessioned2025-02-04T23:15:52Z
dc.date.available2025-02-04T23:15:52Z
dc.date.issued2024
dc.description.abstractIndividuals infected with enteric viruses excrete them in their feces for an extended period, whether symptomatic or asymptomatic. This characteristic, combined with the capability of these viruses to persist in the environment, forms the core of our research. The objective of our study was to investigate the presence of viruses associated with diarrhea and hepatitis: Hepatitis A virus (HAV), Hepatitis E virus (HEV), Norovirus GI (NoV GI), Norovirus GII (NoV GII), and Rotavirus (RV) by RT-real time PCR, in untreated wastewater samples (n=100) collected in the period July 2020 to August 2021 from two low-income neighborhoods in the district of La Plata, Buenos Aires, Argentina. Globally, the percentage of positive samples was 25 % for HEV, 27 % for RV, 14 % for NoV GII, 1 % for NoV GI, and no detectable samples were found for HAV. HEV, RV, and NoV GII were detected in most of the studied months, with the highest detection rates of RV and NoV GII during the winter season. Regarding RV positive samples, the gene encoding the VP8* protein of three samples was sequenced and phylogenetic analysis revealed that the detected strains belonged to genotypes P[8] and P[3]. Additionally, four NoV strains were also genetically characterized by amplifying a fragment corresponding to the ORF-1/ORF-2 junction region. The identified strains were NoV GI.5, NoV GII.4, NoV GII.17 and NoV GII.20. Our results provide relevant information and serve as scientific evidence of the importance of considering wastewater analysis as a feasible strategy to determine the circulation of enteric viruses in the population, with the further benefit of predicting emerging strains. Moreover, this study represents the first report of the circulation of NoV GII.20 and GI.5 genotypes in our country.eng
dc.description.filiationFil: Frydman, Camila Ayelen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; Argentinaspa
dc.description.filiationFil: Miño, S.. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Misiones. Estacion Experimental Agropecuaria Cerro Azul.; Argentinaspa
dc.description.filiationFil: Iglesias, N. G.. Universidad Nacional de Hurlingham.; Argentinaspa
dc.description.filiationFil: Carballeda, J. M.. Universidad Nacional de Hurlingham.; Argentinaspa
dc.description.filiationFil: Simari, Milagros Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentinaspa
dc.description.filiationFil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentinaspa
dc.description.filiationFil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina. Universidad Nacional de Hurlingham.; Argentinaspa
dc.description.filiationFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; Argentispa
dc.description.urihttps://linkinghub.elsevier.com/retrieve/pii/S266676572400019X
dc.format.mimetypeapplication/pdf
dc.identifier.citationFrydman, Camila Ayelen; Miño, S.; Iglesias, N. G.; Carballeda, J. M.; Simari, Milagros Belén; et al. (2024) Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20. Environmental Advances, 15, pp. 1-10
dc.identifier.doihttps://doi.org/10.1016/j.envadv.2024.100501
dc.identifier.issn2666-7657
dc.identifier.urihttps://repositorio.unahur.edu.ar/handle/123456789/741
dc.journal.pagination1-14
dc.journal.titleEnvironmental Advances
dc.journal.volume15
dc.language.isoeng
dc.publisherElsevier
dc.rights.licenseinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.ocde1Ciencias Médicas y de Salud
dc.subject.ocde2Biotecnología en Salud
dc.subject.unahurBiotecnología Aplicada
dc.titleWastewater surveillance of enteric viruses in eastern Argentina : high rates of detection and first report of NoV GI.5 and GII.20
dc.typeinfo:eu-repo/semantics/article
dc.type.oaireinfo:eu-repo/semantics/article
dc.type.snrdinfo:ar-repo/semantics/artículo
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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