Person:
Martinetti Montanari, Jorge Aníbal

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14/05/1976
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Laboratorio de Nanosistemas de Aplicación Biotecnológica (LANSAB)
El Laboratorio de Nanosistemas de Aplicación Biotecnológica (LANSAB) está especializado en la encapsulación de fármacos, cosméticos, tópicos y activos naturales. Líneas de investigación: Nanotecnología aplicada a enfermedades cutáneas (cáncer de piel y leishmaniasis). Nanotecnología aplicada a formulaciones de uso veterinario. Nanotecnología aplicada a la salud del suelo. Desarrollo de nanocosméticos y nanorrepelentes.
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Martinetti Montanari
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Jorge Aníbal
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Author: Bucci, Paula Lorena × Has files: true ×

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    Small extracellular vesicles from iPSC-MSC lose their regenerative potential upon UV-C irradiation
    (Cold Spring Harbor Laboratory Press, 2021) Biani, María Celeste; Lombardi, Antonella; Norris, Alessandra; Bucci, Paula Lorena; la Greca, Alejandro Damián; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo; Martinetti Montanari, Jorge Aníbal; Miriuka, Santiago Gabriel; Luzzani, Carlos Daniel
    Mesenchymal Stem Cells derived from induced Pluripotent Stem cells (iPSC-MSC) have become a promising alternative to classical Mesenchymal Stem Cells in regenerative medicine. Their properties -as immunomodulatory and regenerative capacities-are in part due to the secretion of Extracellular Vesicles (EVs). Small EVs (sEVs) with sizes that range from 50 to 120 nm contain proteins, lipids, and nucleic acids that exert a role in cellular communication. Their content will depend on the cell of origin and its physiological state, thus the message they convey might change in response to changes in cellular conditions. In particular, the DNA damage response (DDR) has been reported to modulate sEVs secretion. In this work, we analyze how UV-C radiation upon iPSC-MSC alter sEVs secretion, cargo and bystander effect. Here, we confirm that UV-C radiation causes DDR in a dose dependent manner. In addition, we found that UV-C induced stress did not modulate the expression of genes that participate in sEVs biogenesis pathway. Consequently, we found that the amount of sEVs secreted by radiated and non-irradiated cells remained stable. However, sEVs from radiated cells were unable to promote cell migration in their target cells. Moreover, a label-free proteomic analysis revealed that UV-C induced DDR produces sEVs with an altered cargo, rich in migration-inhibiting proteins, and resulting in a less stromal-oriented repertoire.